In vitro propagation of ginger (Zingiber officinale Rosco)

An efficient and promising protocol for in vitro propagation of Zingiber officinale Rosco using sprouting buds was established. Sprouting buds were sterilized and cultured onto MS medium supplemented with different growth regulators. Augmentation of MS-medium with 4.5 mg/l BAP recorded the highest percentage of shootlets multiplication. Shootlets were highly rooted on half strength of B5 medium supplemented with 1.0 mg/l NAA. The maximum percentage of acclimatization, hardening and rhizomes production of in vitro derived plants in greenhouse was 80–100%.