|کد مقاله||کد نشریه||سال انتشار||مقاله انگلیسی||ترجمه فارسی||نسخه تمام متن|
|2088053||1545687||2015||7 صفحه PDF||سفارش دهید||دانلود رایگان|
• Affinity matrix technology is useful for detecting antibody-producing cells.
• Intact hapten-specific antibody secreting cells can be isolated.
• Surface biotinylation and hapten-conjugated streptavidin increase signals.
We developed a method to detect and isolate plasma cells that produce antigen-specific antibodies. An affinity matrix of hapten was constructed on a cell surface, and subsequent incubation allowed cells to secrete antibodies. Anti-hapten antibodies preferentially bound to the affinity matrix on the cells from which they were secreted. We showed that the combination of surface biotinylation and streptavidin which was conjugated with a high valence of hapten was suitable for sensitive detection of antibody binding. Using this protocol, anti-hapten plasma cells from immunized mouse spleen were detected and enriched by flow cytometry. This method allows for isolation of intact plasma cells according to the antibody specificity and may be useful for highly efficient and precise analysis of an antibody repertoire.
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Journal: Journal of Immunological Methods - Volume 422, July 2015, Pages 80–86