کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2088214 | 1545703 | 2014 | 6 صفحه PDF | دانلود رایگان |
• Sustained depletion of splenic langerin+ CD8α+ dendritic cells in lang-DTREGFP mice
• No weight loss or toxicity with diphtheria toxin treatment in lang-DTREGFP mice
• Diphtheria toxin treatment of lang-DTREGFP mice does not result in neutrophilia.
Splenic langerin+ CD8α+ dendritic cells (DCs) have exhibited a critical role in cross-priming CD8+ T cell responses. To further study the roles of this DC subset, a protocol for the continuous depletion of langerin+ CD8α+ DCs was established using the pre-existing lang-DTREGFP mouse model. Due to the fast turnover rate of splenic CD8α+ DCs, maintaining the depletion of langerin+ CD8α+ DCs required multiple diphtheria toxin (DT) treatments. We found that prolonged treatment with DT did not cause weight loss, or neutrophilia, as reported in some DT-based depletion models. Therefore, the in vivo depletion of murine langerin+ CD8α+ DCs can be maintained over time to analyse their function during the full course of an immune response.
Journal: Journal of Immunological Methods - Volume 406, April 2014, Pages 104–109