کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2088430 | 1545735 | 2011 | 12 صفحه PDF | دانلود رایگان |
A/J mice bearing the H-2 allele IAk are highly susceptible to autoimmune myocarditis induced with cardiac myosin heavy chain (Myhc)-α 334–352, whereas B10.A mice carrying a similar allele IAk are relatively resistant, suggesting that the generation of Myhc-α-reactive T cell repertoires is influenced by genetic background. To enumerate the precursor frequencies of Myhc-α-specific CD4 T cells, we sought to create IAk tetramers for Myhc-α 334–352. Tetramers were created using approaches that involve covalent tethering of individual peptide sequences or exogenous loading of peptides into empty IAk molecules by peptide-exchange reaction. Using ribonuclease 43–56 tetramers as controls, we demonstrated that by flow cytometry (FC), Myhc-α 334–352 tetramers specifically bind myosin-reactive T cells. CD4 cells isolated from A/J mice immunized with Myhc-α 334–352 were used to optimize conditions for tetramer staining, and neuraminidase treatment prior to tetramer staining permitted the detection of Myhc-α-specific cells ex vivo. The reagents are useful tools for monitoring the frequency of Myhc-α-reactive CD4 cells and to determine their pathogenic potential at a single cell level by FC.
► Created tetramers for MHC class II, IAk allele.
► IAk/tetramers were found useful to enumerate the frequencies of cardiac myosin-specific cells.
► Peptide-exchange reaction allowed us to generate tetramers for multiple peptides.
Journal: Journal of Immunological Methods - Volume 372, Issues 1–2, 30 September 2011, Pages 107–118