کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2088622 1545753 2010 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Generation of monoclonal antibodies against human regulatory T cells
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
پیش نمایش صفحه اول مقاله
Generation of monoclonal antibodies against human regulatory T cells
چکیده انگلیسی

Natural CD4+CD25+Foxp3+ regulatory T cells (Tregs) control the activation of the immune system and therefore have become a major area of research in immunology. The generation of monoclonal antibodies against human Tregs offers the possibility to discover novel Treg-specific or Treg-associated surface markers and to identify targets for a therapeutic modulation of Tregs. Here we present a methodology optimized to efficiently induce and select mAb against human Tregs by repeated immunization of mice with Tregs from a single donor and a differential two-step flow cytometry-based hybridoma screening procedure.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Immunological Methods - Volume 353, Issues 1–2, 28 February 2010, Pages 62–70
نویسندگان
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