کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2088624 1545753 2010 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A novel composite immunotoxin that suppresses rabies virus production by the infected cells
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
پیش نمایش صفحه اول مقاله
A novel composite immunotoxin that suppresses rabies virus production by the infected cells
چکیده انگلیسی

Using strepavidin as a scaffold, we have assembled a composite immunotoxin that consists of recombinant Pseudomonas exotoxin A subunit (PE38) and recombinant 25-D1.16 Fab fragment which recognizes the SIINFEKL (pOV8) peptide from ovalbumin in association with H-2Kb MHC class I protein. The composite immunotoxin exercises cytotoxicity against H-2Kb+ cells sensitized with pOV8 peptide but not with irrelevant peptide. Specific binding of the immunotoxin to H-2Kb+ cells infected with recombinant rabies virus (RV) expressing pOV8 epitope (RV-pOV8) resulted in the suppression of the production of virus particles by the infected cells. This strategy allows readily produce different immunotoxins with desired specificity by combining various targeting and toxin molecules. The results provide a proof of concept that composite immunotoxins can be utilized as novel immunotherapeutics to stop virus spread in the acute phase of the infection allowing winning time for the development of protective immune response.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Immunological Methods - Volume 353, Issues 1–2, 28 February 2010, Pages 78–86
نویسندگان
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