کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2088733 | 1545754 | 2010 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Immunofluorescence microscopy for imaging of nuclear p63 in human primary keratinocytes: A comparison of antibodies and fixation methods
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوتکنولوژی یا زیستفناوری
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Immunofluorescence microscopy for imaging of nuclear p63 in human primary keratinocytes: A comparison of antibodies and fixation methods Immunofluorescence microscopy for imaging of nuclear p63 in human primary keratinocytes: A comparison of antibodies and fixation methods](/preview/png/2088733.png)
چکیده انگلیسی
The ability of an experimental treatment to induce primitive, undifferentiated stem cells towards an epidermal fate may be tested by comparing the treated stem cells with a positive control, such as primary keratinocytes. In an effort to perfect methods used for this comparison, we tested two commercially available antibodies and three fixation methods to determine which antibody/fixation interaction produced the best immunofluorescent images of the nuclear localization of p63, a canonical marker of epidermal fate, in keratinocytes. Here, we report the methods used, and the experimental outcome.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Immunological Methods - Volume 352, Issues 1–2, 31 January 2010, Pages 174–177
Journal: Journal of Immunological Methods - Volume 352, Issues 1–2, 31 January 2010, Pages 174–177
نویسندگان
Chad D. Markert, Shantaram Bharadwaj, Yuanyuan Zhang, Martin K. Childers, Mark E. Furth,