کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2088743 | 1545746 | 2010 | 9 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: A recombinant human HLA-class I antigen linked to dextran elicits innate and adaptive immune responses A recombinant human HLA-class I antigen linked to dextran elicits innate and adaptive immune responses](/preview/png/2088743.png)
The objective of this study was to produce and evaluate the immunogenic potential of a recombinant HLA-class I antigen linked to dextran. The HLA-A*0201 heavy chain and β2 microglobulin were cloned by PCR amplification of overlapping oligonucleotides and produced in E. coli. These were assembled with a CMV binding peptide motif, the HLA complex was biotinylated and bound by streptavidin coated dextran at a ratio of 24 HLA to 1 dextran molecule (termed Dextramer). Allostimulation of human PBMC in vitro and in vivo immunization of Balb c mice with the HLA-A*0201 construct elicited CD4+ and CD8+ T cell proliferative responses, IgG specific antibodies in mice and in human T cell proliferation and APOBEC3G mRNA. These adaptive and innate immune responses induced by a novel recombinant HLA construct in human cells and mice suggest their application as a potential vaccine candidate against HIV infection.
Journal: Journal of Immunological Methods - Volume 360, Issues 1–2, 31 August 2010, Pages 1–9