کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2088860 | 1545763 | 2009 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Gene gun-supported DNA immunisation of chicken for straightforward production of poxvirus-specific IgY antibodies
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موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوتکنولوژی یا زیستفناوری
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![عکس صفحه اول مقاله: Gene gun-supported DNA immunisation of chicken for straightforward production of poxvirus-specific IgY antibodies Gene gun-supported DNA immunisation of chicken for straightforward production of poxvirus-specific IgY antibodies](/preview/png/2088860.png)
چکیده انگلیسی
To simplify this procedure, IgY antibodies specific for poxviral proteins (F1L and p28) were generated by immunisation of chickens, because antibody retrieval from eggs allows the non-invasive generation of huge amounts of antibodies. The main intentions were (i) to decrease invasiveness, (ii) to immunise with native forms of proteins and (iii) to circumvent previous protein expression and purification. Therefore, chicken were immunised with DNA expression vectors coding for conserved domains of the selected proteins delivered for the first time by a gene gun. Four weeks after initial immunisation specific antibodies were found in the egg yolk as proven by immunofluorescence staining of poxvirus-infected cells. The specific IgY titre rose to 1:80,000 and was stable for more than 120Â days. With this investigation we present an universal procedure for IgY design and production that can be applied for various issues in the future.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Immunological Methods - Volume 341, Issues 1â2, 28 February 2009, Pages 146-153
Journal: Journal of Immunological Methods - Volume 341, Issues 1â2, 28 February 2009, Pages 146-153
نویسندگان
Peter T. Witkowski, Daniel R. Bourquain, Oliver Hohn, Rüdiger Schade, Andreas Nitsche,