کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2088937 1545771 2008 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
T cell acquisition of APC membrane can impact interpretation of adoptive transfer experiments using CD45 congenic mouse strains
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
پیش نمایش صفحه اول مقاله
T cell acquisition of APC membrane can impact interpretation of adoptive transfer experiments using CD45 congenic mouse strains
چکیده انگلیسی

Congenic mouse strains bearing allelic variants of CD45 are often used in adoptive transfer experiments. Here, we report that immune CD8+ splenocytes of donor origin acquired the recipient's congenic CD45 marker during interaction with antigen-bearing cells, presumably as a result of membrane transfer upon dissolution of the immunological synapse. Acquisition of recipient marker by donor cells was most prominent after in vitro incubation with peptide for intracellular cytokine staining, where most of the antigen-bearing splenocytes are of recipient origin. In consequence, when antibodies against the recipient's congenic marker were used to distinguish donor and recipient populations, donor origin cells were incorrectly interpreted as being of recipient origin. This phenomenon may cause problems for interpretation of data in adoptive transfer experiments primarily when (a) staining for the recipient's congenic marker and (b) identifying antigen-specific populations by staining for intracellular cytokine.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Immunological Methods - Volume 330, Issues 1–2, 31 January 2008, Pages 137–145
نویسندگان
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