کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2088975 1645471 2008 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
In vitro neutralization of equid herpesvirus 1 mediated by recombinant antibodies
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
پیش نمایش صفحه اول مقاله
In vitro neutralization of equid herpesvirus 1 mediated by recombinant antibodies
چکیده انگلیسی

A phage antibody display library of single chain fragment variables (scFv) was applied to develop anti-equid herpesvirus-1 (EHV-1) glycoprotein D (gD) neutralizing antibodies. To enrich for specific scFvs, the phage antibody library was panned against epitope derived from the N-terminal part of EHV-1 gD. Unique clones were differentiated by BstNI fingerprinting and further characterized by sequencing and immunoreactivity. The neutralizing effect of each clone was assessed by plaque reduction assay. Three clones with neutralizing effect were isolated.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Immunological Methods - Volume 333, Issues 1–2, 20 April 2008, Pages 186–191
نویسندگان
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