کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2089052 1545773 2007 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A new simple multi-well plate-based assay for monocyte differentiation using human monocytic leukemia THP-1 cells
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
پیش نمایش صفحه اول مقاله
A new simple multi-well plate-based assay for monocyte differentiation using human monocytic leukemia THP-1 cells
چکیده انگلیسی

A new simple multi-well plate-based assay to determine monocyte differentiation-inducing activity was developed. It is based on the increase of macrophage adherence after the induction of monocyte differentiation. The assay was conducted using a human monocytic leukemia cell line, THP-1, treated with either 12-O-tetradecanoylphorbol 13-acetate or cytokines, such as tumor necrosis factor-α and interferon-γ. This method is sensitive and easy to perform, especially in case of analyzing a large number of samples.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Immunological Methods - Volume 328, Issues 1–2, 1 December 2007, Pages 215–219
نویسندگان
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