کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2089518 1545792 2006 13 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Comparison study of streptavidin-coated microtitration plates
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
پیش نمایش صفحه اول مقاله
Comparison study of streptavidin-coated microtitration plates
چکیده انگلیسی

Streptavidin (SAv)-coated 96-well microtitration plates from commercial suppliers (six plates) and our laboratory (two plates) were tested with respect to their binding capacity for small and large molecules, leaching, well-to-well variation and immunoassay performance. The binding capacities for europium-labelled biotin (Eu-biotin) varied from 4.4 to above 150 pmol/well depending on the plate, and for biotinylated monoclonal antibody from 1.2 to 6.4 pmol/well (190–1030 ng). Incubation for 1 h in regular immunoassay buffer resulted in leaching of 0.6–76 ng of SAv from unwashed wells and 0.5–60 ng from pre-washed wells. The desorbed quantity represented up to 3.4% of the maximal Eu-biotin binding capacity. Coating-related variation of 96 wells (CV% values) ranged from 1.2% to 8.0% when tested with respect to maximum binding capacity. Immunofluorometric assay for TSH as well as enzyme immunoassays for CA125 and PSA revealed immunoassay-related performance of the plates, regarding signal levels, variation and non-specific binding characteristics. Non-specific binding and variation tended to increase in the highest capacity plates, whereas some low capacity plates fulfilled the assay requirements optimally. Good performance with respect to capacity, coating homogeneity or leaching was not by definition reflected in the performance of two-site heterogeneous immunoassays.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Immunological Methods - Volume 308, Issues 1–2, 20 January 2006, Pages 203–215
نویسندگان
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