|کد مقاله||کد نشریه||سال انتشار||مقاله انگلیسی||ترجمه فارسی||نسخه تمام متن|
|2089651||1545913||2016||4 صفحه PDF||سفارش دهید||دانلود رایگان|
• Illumina genomic libraries were made using one nanogram or less of DNA.
• Sequencing reads from ultra-low input resulted in quality genome assemblies.
• The Ovation Ultralow method produced the best libraries for Illumina sequencing.
Illumina library preparation methods for ultra-low input amounts were compared using genomic DNA from two foodborne parasites (Angiostrongylus cantonensis and Cyclospora cayetanensis) as examples. The Ovation Ultralow method resulted in libraries with the highest concentration and produced quality sequencing data, even when the input DNA was in the picogram range.
Journal: Journal of Microbiological Methods - Volume 130, November 2016, Pages 23–26