A strip array for spoligotyping of Mycobacterium tuberculosis complex isolates

• A strip array for spoligotyping of MTBC was developed.
• This method is more rapid and convenient than the traditional spoligotyping method.
• The results of 211 MTBC isolates were fully concordant with the traditional method.

A novel strip array was developed for a nine-spacer spoligotyping scheme of Mycobacterium tuberculosis complex (MTBC). The new method was evaluated using 211 MTBC isolates and the results were fully concordant with the traditional spoligotyping approach. The strip array proved to be rapid and convenient for spoligotyping of MTBC.