کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2091987 | 1545986 | 2016 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Functions of Saccharomyces cerevisiae Ecm27p, a putative Na+/Ca2+ exchanger, in calcium homeostasis, carbohydrate storage and cell cycle reentry from the quiescent phase
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوتکنولوژی یا زیستفناوری
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چکیده انگلیسی
The quiescent phase of the cell cycle is of fundamental importance for fungi, yet our understanding of this phase of the cycle is much less well understood than the mitotic cell cycle. We found that the ECM27 gene, which encodes a Na+/Ca2+ exchanger, is responsible for influx of calcium from the extracellular space and release from intracellular stores during membrane stress. Wild type cells increase total Ca2+ in quiescence but cells lacking ECM27 gene fail to do so and are defective in cell cycle reentry from the quiescent phase. ecm27Î cells are also defective in maintaining trehalose levels throughout this phase. Addition of high levels of CaCl2 to the growth medium can increase total cellular calcium in ecm27Î cells during quiescence and can also restore trehalose levels as well as partially restore ability of cells to reenter the mitotic cell cycle. ecm27Î cells also have altered glycogen levels in exponentially growing cells. Our results show that Ecm27p and Ca2+ play roles in maintaining a high level of trehalose in quiescent cells, which in turn is important in the ability of cells to rapidly return to proliferation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Microbiological Research - Volumes 186â187, MayâJune 2016, Pages 81-89
Journal: Microbiological Research - Volumes 186â187, MayâJune 2016, Pages 81-89
نویسندگان
Rachel Klukovich, William E. Courchesne,