کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2092384 1081787 2013 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Role of plnB gene in the regulation of bacteriocin production in Lactobacillus paraplantarum L-XM1
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
پیش نمایش صفحه اول مقاله
Role of plnB gene in the regulation of bacteriocin production in Lactobacillus paraplantarum L-XM1
چکیده انگلیسی

Homologues of plnB gene have been shown to participate in regulation of bacteriocin production through quorum sensing system in other organisms, to investigate the possible role of plnB gene in Lactobacillus paraplantarum L-XM1, we cloned and insertionally inactivated the plnB gene. The plnB knockout mutant ΔplnB21 showed loss of bacteriocin production, its Bac+ phenotype could not be restored even after the addition of PlnA. Furthermore, reverse transcription-PCR analysis from total RNA preparations showed that the bacteriocin structural genes of the plnEF and plnJK were not transcribed in the plnB knockout mutant compared with the wild-type strain. It was therefore concluded that plnB is invovled in a quorum sensing based bacteriocin production. This is the first demonstration of a role for plnB by gene knockout in L. paraplantarum.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Microbiological Research - Volume 168, Issue 5, 12 June 2013, Pages 305–310
نویسندگان
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