کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2092936 1081837 2006 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Comparison of the SYBR Green and the hybridization probe format for real-time PCR detection of HHV-6
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
پیش نمایش صفحه اول مقاله
Comparison of the SYBR Green and the hybridization probe format for real-time PCR detection of HHV-6
چکیده انگلیسی

SummaryA comparative study was conducted of a novel real-time quantitative PCR test (LightCycler System) with FastStart DNA MasterPLUS SYBR Green I dye to detect DNA of human herpes virus 6 (HHV-6). Results were compared with those of a real-time quantitative PCR with hybridization probe (HP) formats using the fluorescence resonance energy transfer method, and with those of a single qualitative PCR test. The detection limit of the test with SYBR Green I dye was 20 copies of the virus, similar to that of the other two tests. The reproducibility was satisfactory. The new test has the same advantages as real-time PCR with HP formats and offers a greater versatility at lower cost.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Microbiological Research - Volume 161, Issue 2, 13 February 2006, Pages 158–163
نویسندگان
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