کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2093099 1081923 2010 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Identification and characterization of CcCTR1, a copper uptake transporter-like gene, in Coprinopsis cinerea
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
پیش نمایش صفحه اول مقاله
Identification and characterization of CcCTR1, a copper uptake transporter-like gene, in Coprinopsis cinerea
چکیده انگلیسی

SummaryCopper (Cu) is an essential element for the physiological function of organisms. In basidiomycetes, Cu is necessary for the production of phenol oxidase enzymes such as laccase and tyrosinase. We isolated and characterized two genes, CcCTR1 and -2, from the model basidiomycete Coprinopsis cinerea. CcCTR1 and -2 showed similarity to the Cu transporter CTR1 in Saccharomyces cerevisiae. Both CcCTRs had a MLxxM motif that is conserved in other CTR homologs. The addition of Cu to a liquid culture of C. cinerea decreased the mRNA accumulation of CcCTR1 and -2. Heterologous expression of CcCTR1 in S. cerevisiae increased Cu sensitivity, suggesting that CcCTR1 is a Cu uptake transporter. Together, these results suggest that CcCTR1 plays an important role in Cu accumulation in C. cinerea.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Microbiological Research - Volume 165, Issue 4, 30 May 2010, Pages 276–287
نویسندگان
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