کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2093294 | 1081951 | 2016 | 16 صفحه PDF | دانلود رایگان |
• ERα+ and ERα− breast cancer stem cells share a quiescent cancer stem cell pool
• Single-cell analysis identified distinct cancer stem cell populations in breast cancer
• Identified ERα+ breast cancer cell populations were hierarchically organized
• Switch-like and gradual transitions exist between ERα+ stem and progenitor pools
SummaryThe identification of breast cancer cell subpopulations featuring truly malignant stem cell qualities is a challenge due to the complexity of the disease and lack of general markers. By combining extensive single-cell gene expression profiling with three functional strategies for cancer stem cell enrichment including anchorage-independent culture, hypoxia, and analyses of low-proliferative, label-retaining cells derived from mammospheres, we identified distinct stem cell clusters in breast cancer. Estrogen receptor (ER)α+ tumors featured a clear hierarchical organization with switch-like and gradual transitions between different clusters, illustrating how breast cancer cells transfer between discrete differentiation states in a sequential manner. ERα− breast cancer showed less prominent clustering but shared a quiescent cancer stem cell pool with ERα+ cancer. The cellular organization model was supported by single-cell data from primary tumors. The findings allow us to understand the organization of breast cancers at the single-cell level, thereby permitting better identification and targeting of cancer stem cells.
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Journal: - Volume 6, Issue 1, 12 January 2016, Pages 121–136