کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2093314 1081952 2016 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Dnd Is a Critical Specifier of Primordial Germ Cells in the Medaka Fish
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
پیش نمایش صفحه اول مقاله
Dnd Is a Critical Specifier of Primordial Germ Cells in the Medaka Fish
چکیده انگلیسی


• The medaka RNA-binding protein Dnd specifies primordial germ cells
• Cells from medaka cleavage embryos can be singly cultured for lineage tracing
• The dnd RNA forms particles as a new mechanism for asymmetric segregation
• These findings offer new insights into PGC specification and manipulation

SummaryPrimordial germ cell (PGC) specification occurs early in development. PGC specifiers have been identified in Drosophila, mouse, and human but remained elusive in most animals. Here we identify the RNA-binding protein Dnd as a critical PGC specifier in the medaka fish (Oryzias latipes). Dnd depletion specifically abolished PGCs, and its overexpression boosted PGCs. We established a single-cell culture procedure enabling lineage tracing in vitro. We show that individual blastomeres from cleavage embryos at the 32- and 64-cell stages are capable of PGC production in culture. Importantly, Dnd overexpression increases PGCs via increasing PGC precursors. Strikingly, dnd RNA forms prominent particles that segregate asymmetrically. Dnd concentrates in germ plasm and stabilizes germ plasm RNA. Therefore, Dnd is a critical specifier of fish PGCs and utilizes particle partition as a previously unidentified mechanism for asymmetric segregation. These findings offer insights into PGC specification and manipulation in medaka as a lower vertebrate model.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 6, Issue 3, 8 March 2016, Pages 411–421
نویسندگان
, , , , , , , , ,