کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2093345 1081954 2014 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Induced Neural Stem Cells Achieve Long-Term Survival and Functional Integration in the Adult Mouse Brain
ترجمه فارسی عنوان
سلول های بنیادی عصبی منجر به دستیابی به بقای بلند مدت و ادغام عملکرد در مغز بزرگسالان می شود.
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
چکیده انگلیسی


• In vivo long-term survival of transplanted induced neural stem cells
• Lack of tumorigenic outgrowth
• In vivo multilineage differentiation of transplanted iNSCs
• Functional integration, synapse formation, and electrophysiological activity

SummaryDifferentiated cells can be converted directly into multipotent neural stem cells (i.e., induced neural stem cells [iNSCs]). iNSCs offer an attractive alternative to induced pluripotent stem cell (iPSC) technology with regard to regenerative therapies. Here, we show an in vivo long-term analysis of transplanted iNSCs in the adult mouse brain. iNSCs showed sound in vivo long-term survival rates without graft overgrowths. The cells displayed a neural multilineage potential with a clear bias toward astrocytes and a permanent downregulation of progenitor and cell-cycle markers, indicating that iNSCs are not predisposed to tumor formation. Furthermore, the formation of synaptic connections as well as neuronal and glial electrophysiological properties demonstrated that differentiated iNSCs migrated, functionally integrated, and interacted with the existing neuronal circuitry. We conclude that iNSC long-term transplantation is a safe procedure; moreover, it might represent an interesting tool for future personalized regenerative applications.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 3, Issue 3, 9 September 2014, Pages 423–431
نویسندگان
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