کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2093461 1081960 2014 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Rapid Cellular Phenotyping of Human Pluripotent Stem Cell-Derived Cardiomyocytes using a Genetically Encoded Fluorescent Voltage Sensor
ترجمه فارسی عنوان
فنوتیپ سریع سلولی از کاردیومیوسیت های سلول های بنیادی پلورپوفت انسانی با استفاده از سنسور ولتاژ فلورسنت کد گذاری شده ژنتیکی
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
چکیده انگلیسی


• Genetic introduction of a fluorescent voltage sensor into hESC-derived cardiomyocytes
• High-throughput electrophysiological analysis of hESC-derived cardiomyocytes
• Demonstration of potential of a fluorescent voltage reporter in drug screening assays

SummaryIn addition to their promise in regenerative medicine, pluripotent stem cells have proved to be faithful models of many human diseases. In particular, patient-specific stem cell-derived cardiomyocytes recapitulate key features of several life-threatening cardiac arrhythmia syndromes. For both modeling and regenerative approaches, phenotyping of stem cell-derived tissues is critical. Cellular phenotyping has largely relied upon expression of lineage markers rather than physiologic attributes. This is especially true for cardiomyocytes, in part because electrophysiological recordings are labor intensive. Likewise, most optical voltage indicators suffer from phototoxicity, which damages cells and degrades signal quality. Here we present the use of a genetically encoded fluorescent voltage indicator, ArcLight, which we demonstrate can faithfully report transmembrane potentials in human stem cell-derived cardiomyocytes. We demonstrate the application of this fluorescent sensor in high-throughput, serial phenotyping of differentiating cardiomyocyte populations and in screening for drug-induced cardiotoxicity.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 2, Issue 2, 11 February 2014, Pages 163–170
نویسندگان
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