کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2093465 1081960 2014 14 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Characterization of Human Induced Pluripotent Stem Cell-Derived Retinal Pigment Epithelium Cell Sheets Aiming for Clinical Application
ترجمه فارسی عنوان
خصوصیات سلول های سلول اپیتلیوم رنگدانه سلول های بنیادی تکثیر یافته سلول های بنیادی منجر به کاربرد بالینی
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
چکیده انگلیسی


• hiPSC-RPE cell sheets show characteristics similar to those of native RPE
• A hiPSC-RPE sheet is sufficient to transplant a patient
• All hiPSC-RPEs show gene-expression patterns similar to native RPE
• Autologous nonhuman primate iPSC-RPE cell sheets show no rejection or tumor

SummaryAge-related macular degeneration (AMD) causes severe visual impairment due in part to age-dependent impairment of retinal pigment epithelium (RPE). It has been suggested that autologous human induced pluripotent stem cells (hiPSCs) may represent a useful cell source for the generation of graft RPE. We generated hiPSC-derived RPE (hiPSC-RPE) cell sheets optimized to meet clinical use requirements, including quality, quantity, consistency, and safety. These cell sheets are generated as a monolayer of cells without any artificial scaffolds, express typical RPE markers, form tight junctions that exhibit polarized secretion of growth factors, and show phagocytotic ability and gene-expression patterns similar to those of native RPE. Additionally, upon transplantation, autologous nonhuman primate iPSC-RPE cell sheets showed no immune rejection or tumor formation. These results suggest that autologous hiPSC-RPE cell sheets may serve as a useful form of graft for use in tissue replacement therapy for AMD.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 2, Issue 2, 11 February 2014, Pages 205–218
نویسندگان
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