کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2093612 | 1081969 | 2015 | 11 صفحه PDF | دانلود رایگان |
• Non-invasive characterization of cardiomyocytes using video motion analysis
• Assessment of single-cell, monolayer, or tissue cardiomyocytes in standard plates
• Drug screening by a label-free, contact-free imaging assay
SummaryWe present a non-invasive method to characterize the function of pluripotent stem-cell-derived cardiomyocytes based on video microscopy and image analysis. The platform, called Pulse, generates automated measurements of beating frequency, beat duration, amplitude, and beat-to-beat variation based on motion analysis of phase-contrast images captured at a fast frame rate. Using Pulse, we demonstrate recapitulation of drug effects in stem-cell-derived cardiomyocytes without the use of exogenous labels and show that our platform can be used for high-throughput cardiotoxicity drug screening and studying physiologically relevant phenotypes.
Graphical AbstractFigure optionsDownload as PowerPoint slide
Journal: - Volume 4, Issue 4, 14 April 2015, Pages 621–631