کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2093632 1081970 2014 14 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Isolation of Human Induced Pluripotent Stem Cell-Derived Dopaminergic Progenitors by Cell Sorting for Successful Transplantation
ترجمه فارسی عنوان
جداسازی پیش سازهای دوپامینرژیک سلول های بنیادی مولکولی منجر به سلول های بنیادی توسط سلول های مرتب سازی برای پیوند موفقیت آمیز
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
چکیده انگلیسی


• DA neurons were highly efficiently induced from iPSCs on xeno-free laminin fragment
• DA progenitors were enriched by sorting of CORIN+ cells
• CORIN+ cell grafts resulted in good DA neuron survival without tumor formation
• NURR1+ cell-dominant stage exhibited the best survival and function as DA neurons

SummaryHuman induced pluripotent stem cells (iPSCs) can provide a promising source of midbrain dopaminergic (DA) neurons for cell replacement therapy for Parkinson’s disease. However, iPSC-derived donor cells inevitably contain tumorigenic or inappropriate cells. Here, we show that human iPSC-derived DA progenitor cells can be efficiently isolated by cell sorting using a floor plate marker, CORIN. We induced DA neurons using scalable culture conditions on human laminin fragment, and the sorted CORIN+ cells expressed the midbrain DA progenitor markers, FOXA2 and LMX1A. When transplanted into 6-OHDA-lesioned rats, the CORIN+ cells survived and differentiated into midbrain DA neurons in vivo, resulting in significant improvement of the motor behavior, without tumor formation. In particular, the CORIN+ cells in a NURR1+ cell-dominant stage exhibited the best survival and function as DA neurons. Our method is a favorable strategy in terms of scalability, safety, and efficiency and may be advantageous for clinical application.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 2, Issue 3, 11 March 2014, Pages 337–350
نویسندگان
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