کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2093716 1081974 2015 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
FGF, Insulin, and SMAD Signaling Cooperate for Avian Primordial Germ Cell Self-Renewal
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
پیش نمایش صفحه اول مقاله
FGF, Insulin, and SMAD Signaling Cooperate for Avian Primordial Germ Cell Self-Renewal
چکیده انگلیسی


• Avian primordial germ cell self-renewal is dependent on FGF2, insulin, and Activin A molecules
• BMP4 can replace Activin A in non-clonal growth conditions
• Defined culture medium conditions will facilitate studies of germ cell self-renewal in other vertebrate species

SummaryPrecise self-renewal of the germ cell lineage is fundamental to fertility and reproductive success. The early precursors for the germ lineage, primordial germ cells (PGCs), survive and proliferate in several embryonic locations during their migration to the embryonic gonad. By elucidating the active signaling pathways in migratory PGCs in vivo, we were able to create culture conditions that recapitulate this embryonic germ cell environment. In defined medium conditions without feeder cells, the growth factors FGF2, insulin, and Activin A, signaling through their cognate-signaling pathways, were sufficient for self-renewal of germline-competent PGCs. Forced expression of constitutively active MEK1, AKT, and SMAD3 proteins could replace their respective upstream growth factors. Unexpectedly, we found that BMP4 could replace Activin A in non-clonal growth conditions. These defined medium conditions identify the key molecular pathways required for PGC self-renewal and will facilitate efforts in biobanking of chicken genetic resources and genome editing.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 5, Issue 6, 8 December 2015, Pages 1171–1182
نویسندگان
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