کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2093727 | 1081975 | 2014 | 9 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Drosophila Perlecan Regulates Intestinal Stem Cell Activity via Cell-Matrix Attachment Drosophila Perlecan Regulates Intestinal Stem Cell Activity via Cell-Matrix Attachment](/preview/png/2093727.png)
• Pcan regulates ISC activity and ISC-ECM attachment in Drosophila adult midgut
• Loss of Pcan from ISC, but not other surrounding cells, causes the ISC defects
• Functions of Pcan are independent of EGFR and JAK/STAT signaling pathways
• ISC secrets Pcan to establish a niche for itself
SummaryStem cells require specialized local microenvironments, termed niches, for normal retention, proliferation, and multipotency. Niches are composed of cells together with their associated extracellular matrix (ECM). Currently, the roles of ECM in regulating niche functions are poorly understood. Here, we demonstrate that Perlecan (Pcan), a highly conserved ECM component, controls intestinal stem cell (ISC) activities and ISC-ECM attachment in Drosophila adult posterior midgut. Loss of Pcan from ISCs, but not other surrounding cells, causes ISCs to detach from underlying ECM, lose their identity, and fail to proliferate. These defects are not a result of a loss of epidermal growth factor receptor (EGFR) or Janus kinase/signal transducer and activator of transcription (JAK/STAT) signaling activity but partially depend on integrin signaling activity. We propose that Pcan secreted by ISCs confers niche properties to the adjacent ECM that is required for ISC maintenance of stem cell identity, activity, and anchorage to the niche.
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Journal: - Volume 2, Issue 6, 3 June 2014, Pages 761–769