کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2093791 1081978 2014 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Chemical Conversion of Human Fibroblasts into Functional Schwann Cells
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
پیش نمایش صفحه اول مقاله
Chemical Conversion of Human Fibroblasts into Functional Schwann Cells
چکیده انگلیسی


• Direct conversion of human fibroblasts into Schwann cells
• No introduction of ectopic genes
• Induction of conversion by multikinase inhibitor

SummaryDirect transdifferentiation of somatic cells is a promising approach to obtain patient-specific cells for numerous applications. However, conversion across germ-layer borders often requires ectopic gene expression with unpredictable side effects. Here, we present a gene-free approach that allows efficient conversion of human fibroblasts via a transient progenitor stage into Schwann cells, the major glial cell type of peripheral nerves. Using a multikinase inhibitor, we transdifferentiated fibroblasts into transient neural precursors that were subsequently further differentiated into Schwann cells. The resulting induced Schwann cells (iSCs) expressed numerous Schwann cell-specific proteins and displayed neurosupportive and myelination capacity in vitro. Thus, we established a strategy to obtain mature Schwann cells from human postnatal fibroblasts under chemically defined conditions without the introduction of ectopic genes.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: - Volume 3, Issue 4, 14 October 2014, Pages 539–547
نویسندگان
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