کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2093821 | 1081979 | 2014 | 13 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: LET-418/Mi2 and SPR-5/LSD1 Cooperatively Prevent Somatic Reprogramming of C. elegans Germline Stem Cells LET-418/Mi2 and SPR-5/LSD1 Cooperatively Prevent Somatic Reprogramming of C. elegans Germline Stem Cells](/preview/png/2093821.png)
• SPR-5/LSD1 and LET-418/Mi2 interact to jointly control germ cell status
• C. elegans germ cells reprogram as neurons in spr-5 let-418 mutants
• SPR-5 and LET-418 counteract COMPASS-dependent H3K4 methylation in the germline
• High H3K4me3 levels in germ cells correlate with somatic reprogramming
SummaryThroughout their journey to forming new individuals, germline stem cells must remain totipotent, particularly by maintaining a specific chromatin structure. However, the place epigenetic factors occupy in this process remains elusive. So far, “sensitization” of chromatin by modulation of histone arrangement and/or content was believed to facilitate transcription-factor-induced germ cell reprogramming. Here, we demonstrate that the combined reduction of two epigenetic factors suffices to reprogram C. elegans germ cells. The histone H3K4 demethylase SPR-5/LSD1 and the chromatin remodeler LET-418/Mi2 function together in an early process to maintain germ cell status and act as a barrier to block precocious differentiation. This epigenetic barrier is capable of limiting COMPASS-mediated H3K4 methylation, because elevated H3K4me3 levels correlate with germ cell reprogramming in spr-5; let-418 mutants. Interestingly, germ cells deficient for spr-5 and let-418 mainly reprogram as neurons, suggesting that neuronal fate might be the first to be derepressed in early embryogenesis.
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Journal: - Volume 2, Issue 4, 8 April 2014, Pages 547–559