کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2093861 | 1081983 | 2013 | 10 صفحه PDF | دانلود رایگان |
• Normal human mammary gland luminal progenitors (LPs) have very short telomeres
• LP nuclei selectively exhibit telomere-associated DNA damage responses
• LPs have selectively elevated hTERT expression and telomerase activity
• These LP features may play a role in mammary tissue homeostasis and transformation
Telomeres are essential for genomic integrity, but little is known about their regulation in the normal human mammary gland. We now demonstrate that a phenotypically defined cell population enriched in luminal progenitors (LPs) is characterized by unusually short telomeres independently of donor age. Furthermore, we find that multiple DNA damage response proteins colocalize with telomeres in >95% of LPs but in <5% of basal cells. Paradoxically, 25% of LPs are still capable of exhibiting robust clonogenic activity in vitro. This may be partially explained by the elevated telomerase activity that was also seen only in LPs. Interestingly, this potential telomere salvage mechanism declines with age. Our findings thus reveal marked differences in the telomere biology of different subsets of primitive normal human mammary cells. The chronically dysfunctional telomeres unique to LPs have potentially important implications for normal mammary tissue homeostasis as well as the development of certain breast cancers.
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Journal: - Volume 1, Issue 1, 4 June 2013, Pages 28–37