کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2094790 1082052 2016 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Use of Aloe vera–based extender for chilling and freezing collared peccary (Pecari tajacu) semen
ترجمه فارسی عنوان
استفاده از الیاف مبتنی بر آلوئه ورا برای سرد کردن و انجماد مایع منی (Pecari tajacu)
کلمات کلیدی
Tayassu tajacu؛ سرد شدن اسپرم؛ انجماد اسپرم؛ آلوئه ورا؛ Cryoprotectant
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
چکیده انگلیسی

As an alternative for the conservation of collared peccary semen, this research aims at evaluating the use of Aloe vera (AV) extract as a cryoprotectant for semen chilling and freezing. Five ejaculates were divided in two aliquots that were diluted in Tris plus egg yolk (EY; 20%) or AV extract (20%) and chilled at 5 °C. In both treatments, an adequate semen conservation was achieved and values closer to 40% motile sperm with viability and osmotic response ranging from 20% to 40%, and normal morphology of 80% were found after 36 hours of storage. Moreover, 12 other ejaculates were diluted in Tris plus EY (20%) or AV extract (5, 10, or 20%) and glycerol (3%). Samples were frozen in liquid nitrogen and thawed after 1 week. After thawing, all the treatments containing EY or AV provided similar values for sperm morphology, viability, osmotic response, membrane integrity, sperm motility, amplitude of lateral head, beat cross frequency, and rapid, low, and static subpopulations, but the highest values for straightness and the lowest values for curvilinear velocity were found using 20% AV (P < 0.05). In conclusion, we found that AV extract at a 20% concentration could be used as an alternative substitute to EY in the formulation of Tris extenders for collared peccaries' semen chilling or freezing.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Theriogenology - Volume 85, Issue 8, May 2016, Pages 1432–1438
نویسندگان
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