کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2094815 1082053 2016 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Cerium dioxide nanoparticles did not alter the functional and morphologic characteristics of ram sperm during short-term exposure
ترجمه فارسی عنوان
نانوذرات دی اکسید سریم ویژگی های عملکردی و مورفولوژیکی اسپرم موش را در طی مواجهه کوتاه مدت تغییر نمی دهد
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
چکیده انگلیسی
The aim of the study was to investigate the interaction and the short-term effects of increasing doses of cerium dioxide nanoparticles (CeO2 NPs) on ram spermatozoa, stored at 4 °C for up to 24 hours, on the main functional and kinematic parameters. Spermatozoa were incubated with 0, 22, 44, and 220 μg/mL of CeO2 NPs at 4 °C and submitted at 0, 2, and 24 hours to the following analyses: (1) intracellular uptake of CeO2 NPs by the spermatozoa; (2) kinematic parameters; (3) acrosome and membrane integrity; (4) integrity of DNA; (5) mitochondrial activity; (6) ROS production. The results indicated that the exposure of spermatozoa to increasing doses of nanoceria was well tolerated. No intracellular uptake of NPs by the cells was observed and both kinematic parameters and status of the membranes were not affected by the incubation with NPs (P > 0.05). Moreover, no influence on the redox status of spermatozoa and on the levels of fragmentation of DNA was reported among groups at any time (P > 0.05). The data collected provide new information about the impact of CeO2 NPs on the male gamete in large animal model and could open future perspectives about their biomedical use in the assisted reproductive techniques.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Theriogenology - Volume 85, Issue 7, 15 April 2016, Pages 1274-1281.e3
نویسندگان
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