کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2095260 1082089 2014 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
CDC20 downregulation impairs spindle morphology and causes reduced first polar body emission during bovine oocyte maturation
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
CDC20 downregulation impairs spindle morphology and causes reduced first polar body emission during bovine oocyte maturation
چکیده انگلیسی

The cell division cycle protein 20 (CDC20) is an essential regulator of cell division, encoded by the CDC20 gene. However, the role of CDC20 in bovine oocyte maturation is unknown. In this study, CDC20 morpholino antisense oligonucleotides (MOs) were microinjected into the cytoplasm of bovine oocytes to block the translation of CDC20 mRNA. CDC20 downregulation significantly reduced the rate of first polar body emission (PB1). Further analysis indicated that oocytes treated with CDC20 MO arrested before or at meiotic stage I with abnormal spindles. To further confirm the functions of CDC20 during oocyte meiotic division, CDC20 MOs were microinjected into oocytes together with a supplementary PB1. The results showed that newly synthesized CDC20 was not necessary at the meiosis II-to-anaphase II transition. Our data suggest that CDC20 is required for spindle assembly, chromosomal segregation, and PB1 extrusion during bovine oocyte maturation.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Theriogenology - Volume 81, Issue 4, 1 March 2014, Pages 535–544
نویسندگان
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