کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2095554 1082126 2011 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Evidence for the fragmentation of VtgAb LvH in common dentex (Dentex dentex), a marine pelagophil teleost
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
Evidence for the fragmentation of VtgAb LvH in common dentex (Dentex dentex), a marine pelagophil teleost
چکیده انگلیسی
Since the timing of the lipovitellin heavy chain (LvH) of vitellogenin Ab (VtgAb) degradation during marine teleosts ontogeny is poorly understood, the current study was planned to address some aspects of this shortcoming. Fertilized eggs (before epiboly) of Dentex dentex were analysed. Vtg-derived proteins, that appeared as 6 protein bands after SDS-PAGE, were purified using specific ion exchange chromatography. Five bands (i.e., ∼78, ∼57, ∼22, ∼19, and ∼17 kDa) were merely stained with Coomassie Blue and one band (i.e., ∼16 kDa) only with Periodic Acid Schiff (PAS). The ∼16 kDa protein band was subjected to a mass spectrometry-based sequencing. These results showed that the ∼16 kDa protein band contains a mixture of “LvH-Ab” derivatives with the same molecular weight. The specific staining system (i.e., PAS) also revealed the glycosylation of some of the LvH-Ab fragments inside the ∼16 kDa protein band. The study provides new data about fragmentation of the LvH-Ab in marine pelagophil teleosts.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Theriogenology - Volume 76, Issue 1, 1 July 2011, Pages 110-114
نویسندگان
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