کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2095554 | 1082126 | 2011 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Evidence for the fragmentation of VtgAb LvH in common dentex (Dentex dentex), a marine pelagophil teleost
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موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
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چکیده انگلیسی
Since the timing of the lipovitellin heavy chain (LvH) of vitellogenin Ab (VtgAb) degradation during marine teleosts ontogeny is poorly understood, the current study was planned to address some aspects of this shortcoming. Fertilized eggs (before epiboly) of Dentex dentex were analysed. Vtg-derived proteins, that appeared as 6 protein bands after SDS-PAGE, were purified using specific ion exchange chromatography. Five bands (i.e., â¼78, â¼57, â¼22, â¼19, and â¼17 kDa) were merely stained with Coomassie Blue and one band (i.e., â¼16 kDa) only with Periodic Acid Schiff (PAS). The â¼16 kDa protein band was subjected to a mass spectrometry-based sequencing. These results showed that the â¼16 kDa protein band contains a mixture of “LvH-Ab” derivatives with the same molecular weight. The specific staining system (i.e., PAS) also revealed the glycosylation of some of the LvH-Ab fragments inside the â¼16 kDa protein band. The study provides new data about fragmentation of the LvH-Ab in marine pelagophil teleosts.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Theriogenology - Volume 76, Issue 1, 1 July 2011, Pages 110-114
Journal: Theriogenology - Volume 76, Issue 1, 1 July 2011, Pages 110-114
نویسندگان
S.-M. Samaee, A. Estévez,