کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2096871 | 1082183 | 2005 | 15 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Influence of extender, freezing rate, and thawing rate on post-thaw motility, viability and morphology of coyote (Canis latrans) spermatozoa Influence of extender, freezing rate, and thawing rate on post-thaw motility, viability and morphology of coyote (Canis latrans) spermatozoa](/preview/png/2096871.png)
The objective of this study was to examine the post-thaw effects of three cryoprotective extenders (Tris–fructose–citric acid extender, Tris–glucose–citric acid extender, and lactose extender), three linear freezing rates (−1, −6, and −20 °C/min), and three thawing rates (37 °C water bath for 120 s, 60 °C water bath for 30 s, and 70 °C water bath for 8 s) on coyote spermatozoa. After thawing, the findings supported that cryopreservation of coyote (Canis latrans) spermatozoa frozen at a moderate freezing rate (−6 °C/min), in either a Tris–fructose or Tris–glucose extender, and thawed at a slow rate (37 °C water bath for 120 s) or moderate rate (60 °C water bath for 30 s), resulted in a more vigorous post-thaw motility (range, 57.5–44.0%) and viability (range, 64–49.6%) with the least amount of morphological and acrosomal abnormalities.
Journal: Theriogenology - Volume 64, Issue 9, December 2005, Pages 1898–1912