کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2108656 | 1083804 | 2007 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Vitamin E suppresses telomerase activity in ovarian cancer cells
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کلمات کلیدی
FBSPMSPCR-ELISAGFPT-TBSTRAPMTSCDDPhTERT - htertOSE - ORtelomere repeat amplification protocol - تلومر تکرار پروتکل تقویتfetal bovine serum - سرم جنین گاوcisplatin - سیس پلاتینphenazine methosulfate - فنزین متوسولفاتTelomerase inhibition - مهار تلومرازVitamin E - ویتامین E Vitamin E acetate - ویتامین E استاتgreen fluorescent protein - پروتئین فلورسنت سبز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Background: Dietary factors influence tumor formation and progression. Vitamin E is a dietary anti-oxidant capable of eliminating free radical damage, inducing apoptosis and decreasing oncogene expression. Therefore, Vitamin E may be a strong candidate for cancer prevention and/or chemotherapeutic intervention. Since telomerase, a ribonucleoprotein uniquely expressed in over 95% of cancers, plays an important role in cellular immortalization, cell growth and tumor progression, the present study investigated the effects of Vitamin E on telomerase activity in human ovarian cancer. Methods: Normal and malignant ovarian surface epithelial (OSE) cells were cultured with and without d-alpha tocopheryl acetate (Vitamin E). MTS and Western immunoblot assays were used to examine the effect of Vitamin E on cell growth, survival and cytotoxicity. PCR-ELISA, RT-PCR and luciferase reporter assays were performed to determine the effect of Vitamin E on telomerase activity. Results: Vitamin E suppressed endogenous telomerase activity in ovarian cancer cells, but had no similar effects in telomerase-negative normal OSE cells. Vitamin E also reduced hTERT-mRNA transcript levels and reduced hTERT promoter activity maximally targeting the â976 to â578Â bp promoter regions. In addition, Vitamin E improved cisplatin-mediated cytotoxicity as evidenced by reduced cancer cell growth and increased cleaved caspase 3 activity. In contrast, Vitamin E protected telomerase-negative OSE cells from cisplatin-mediated cytotoxicity as evidenced by decreased cleaved caspase 3 activity. Conclusion: Our data suggest that, by suppressing telomerase activity, Vitamin E may be an important protective agent against ovarian cancer cell growth as well as a potentially effective therapeutic adjuvant.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cancer Detection and Prevention - Volume 31, Issue 2, 2007, Pages 119-128
Journal: Cancer Detection and Prevention - Volume 31, Issue 2, 2007, Pages 119-128
نویسندگان
Yira MS, Shirrin BS, Nancy E. MS, Patricia A. PhD,