کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2108680 1083805 2007 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
CHFR gene is neither mutated nor hypermethylated in ovarian cancer
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی تحقیقات سرطان
پیش نمایش صفحه اول مقاله
CHFR gene is neither mutated nor hypermethylated in ovarian cancer
چکیده انگلیسی

Background: CHFR is a mitotic checkpoint protein that under mitotic stress (caused also by taxanes) delays progression into mitosis. Methods: We looked for CHFR gene (605209) alterations and its promoter hypermethylation in 48 ovarian carcinomas either sensitive or resistant to taxane treatment. We used single-strand conformation polymorphism analysis, sequencing and methylation-specific polymerase chain reaction. Results: Neither mutations in the CHFR gene nor hypermethylation of its promoter region were found. A novel single nucleotide polymorphism (Val154Val) was identified in exon 5 of alternatively spliced transcript. Conclusions: Our results indicate that CHFR gene status cannot serve as a molecular predictor of ovarian cancer sensitivity to taxanes.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cancer Detection and Prevention - Volume 31, Issue 3, 2007, Pages 257–261
نویسندگان
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