کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2108756 | 1083809 | 2006 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Deoxyribonucleic acid content as an indicator of progression of squamous cell carcinogenesis in the esophagus: Comparative analysis on imprint-cytospin and tissue section preparation
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کلمات کلیدی
Genetic instability - بی ثباتی ژنتیکیImage analysis - تجزیه و تحلیل تصویرMolecular analysis - تجزیه و تحلیل مولکولیStatistical analysis - تحلیل آماریTumor differentiation - تمایز تمایزCell cycle regulation - تنظیم چرخه سلولیsurvival data - داده های بقاearly cancer - سرطان اولیهCytometry - سیتومتریClinical parameters - پارامترهای بالینیDNA ploidy - پلویید DNASquamous cell carcinoma - کارسینوم سلول سنگفرشی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Background: The purpose of this study was to explore the potential use of image analysis on tissue sections preparation as a predictive marker of early malignant changes during squamous cell (SC) carcinogenesis in the esophagus. Results of DNA ploidy quantification on formalin-fixed, paraffin-embedded tissue using two different techniques were compared: imprint-cytospin and 6 μm thick tissue sections preparation. Methods: This retrospective study included 26 surgical specimens of squamous cell carcinoma (SCC) from patients who underwent surgery alone at the Department of Surgery in CHUV Hospital in Lausanne between January 1993 and December 2000. We analyzed 53 samples of healthy tissue, 43 tumors and 7 lymph node metastases. Results: Diploid DNA histogram patterns were observed in all histologically healthy tissues, either distant or proximal to the lesion. Aneuploidy was observed in 34 (79%) of 43 carcinomas, namely 24 (75%) of 32 early squamous cell carcinomas and 10 (91%) of 11 advanced carcinomas. DNA content was similar in the different tumor stages, whether patients presented with single or multiple synchronous tumors. All lymph node metastases had similar DNA content as their primary tumor. Conclusions: Early malignant changes in the esophagus are associated with alteration in DNA content, and aneuploidy tends to correlate with progression of invasive SCC. A very good correlation between imprint-cytospin and tissue section analysis was observed. Although each method used here showed advantages and disadvantages; tissue sections preparation provided useful information on aberrant cell-cycle regulation and helped select the optimal treatment for the individual patient along with consideration of other clinical parameters.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cancer Detection and Prevention - Volume 30, Issue 3, 2006, Pages 276-283
Journal: Cancer Detection and Prevention - Volume 30, Issue 3, 2006, Pages 276-283
نویسندگان
Snezana MD, Maria-Chiara MD, Liette RT, Jean-Pierre PhD, Philippe MD,