کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2114048 | 1084514 | 2010 | 10 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Clinically relevant doses of chemotherapy agents reversibly block formation of glioblastoma neurospheres Clinically relevant doses of chemotherapy agents reversibly block formation of glioblastoma neurospheres](/preview/png/2114048.png)
Glioblastoma patients have a poor prognosis, even after surgery, radiotherapy, and chemotherapy with temozolomide or 1,3-bis(2-chloroethy)-1-nitrosourea. We developed an in vitro recovery model using neurosphere cultures to analyze the efficacy of chemotherapy treatments, and tested whether glioblastoma neurosphere-initiating cells are resistant. Concentrations of chemotherapy drugs that inhibit neurosphere formation are similar to clinically relevant doses. Some lines underwent a transient cell cycle arrest and a robust recovery of neurosphere formation. These results indicate that glioblastoma neurospheres can regrow after treatment with chemotherapy drugs. This neurosphere recovery assay will facilitate studies of chemo-resistant subpopulations and methods to enhance glioblastoma therapy.
Journal: Cancer Letters - Volume 296, Issue 2, 28 October 2010, Pages 168–177