کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2114981 1084566 2008 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Selective blockade of T-type Ca2+ channels suppresses human breast cancer cell proliferation
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی تحقیقات سرطان
پیش نمایش صفحه اول مقاله
Selective blockade of T-type Ca2+ channels suppresses human breast cancer cell proliferation
چکیده انگلیسی

We have measured the expression of T-type Ca2+ channel mRNA in breast cancer cell lines (MCF-7 (ERα+) using Western blot and quantitative real-time PCR (Q-RT-PCR). These results revealed that the MCF-7 cells express both α1G and α1H isoforms of T-type Ca2+ channels. In order to further clarify the role of T-type Ca2+ channels in proliferation, we tested the effects of a selective T-type Ca2+ channel inhibitor NNC-55-0396 on cellular proliferation. MCF-7 (ERα+) cellular proliferation was inhibited by the compound. In contrast, NNC-55-0396 at same concentration had no effect on the proliferation of MCF-10A cells, a non-cancer breast epithelial cell line. We also found that message expression of the T-type Ca2+ channels were only expressed in rapidly growing non-confluent cells but not in the cytostatic confluent cells. Knocking down the expression of T-type Ca2+ channels with siRNA targeting both α1G and α1H resulted in growth inhibition as much as 45% ± 5.0 in MCF-7 cells as compared to controls. In conclusion, our results suggest that T-type Ca2+ channel antagonism/silencing may reduce cellular proliferation in mitogenic breast cells.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cancer Letters - Volume 267, Issue 1, 18 August 2008, Pages 116–124
نویسندگان
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