کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2115416 1084594 2007 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Initiation of apoptosis and autophagy by the Bcl-2 antagonist HA14-1
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی تحقیقات سرطان
پیش نمایش صفحه اول مقاله
Initiation of apoptosis and autophagy by the Bcl-2 antagonist HA14-1
چکیده انگلیسی

L1210 murine leukemia cells exposed to an LD90 concentration of the Bcl-2/Bcl-xL antagonist HA14-1 rapidly undergo apoptosis but also develop numerous intracellular vacuoles with double membranes, exhibit enhanced labeling by monodansylcadaverine, and convert the cytosolic protein LC3-I to LC3-II. These are hallmarks of autophagy. Autophagic vacuoles develop rapidly, preceding the appearance of an apoptotic nuclear morphology and can be observed in both non-apoptotic and apoptotic cells. Inhibition of autophagy by the PI 3-kinase inhibitor wortmannin promoted apoptosis; conversely inhibition of caspase-3/7 with zDEVD-fmk promoted autophagy. Neither process was dependent on calcium translocation. These results indicate that pharmacological suppression of Bcl-2 function can mimic the induction of autophagy that can occur following the down-regulation of Bcl-2 expression by molecular approaches.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cancer Letters - Volume 249, Issue 2, 8 May 2007, Pages 294–299
نویسندگان
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