کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2116329 1084835 2013 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Adenovirus vector-mediated expression of TMEM166 inhibits human cancer cell growth by autophagy and apoptosis in vitro and in vivo
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی تحقیقات سرطان
پیش نمایش صفحه اول مقاله
Adenovirus vector-mediated expression of TMEM166 inhibits human cancer cell growth by autophagy and apoptosis in vitro and in vivo
چکیده انگلیسی

TMEM166 is a novel programmed cell death-related molecule. In this report, we constructed a recombinant adenovirus 5-TMEM166 vector (Ad5-TMEM166) and evaluated its expression and anti-tumor activities in vitro and in vivo. Cell viability analysis revealed that the adenovirus-mediated increase of TMEM166 inhibited tumor cell growth in a dose- and time-dependent manner. This inhibitory effect was mediated by both autophagy (via inhibition of mTOR and activation of p70S6K) and apoptosis (via caspase-3 activation), both of which contributed to cell death and suppression of tumorigenicity. Our data indicated that Ad5-TMEM166 may be a novel gene therapy candidate for cancer.


► Ad5-TMEM166 inhibits tumor cell growth in vitro and in vivo.
► Ad5-TMEM166 induces cancer cell autophagy via mTOR/p70S6K pathway.
► Ad5-TMEM166 induces cancer cell apoptosis via caspase-3 activation.
► Ad5-TMEM166 may be a novel gene therapy candidate for cancer.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cancer Letters - Volume 328, Issue 1, 1 January 2013, Pages 126–134
نویسندگان
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