کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2116544 | 1084966 | 2009 | 9 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Ex vivo expansion of tumor specific lymphocytes with IL-15 and IL-21 for adoptive immunotherapy in melanoma Ex vivo expansion of tumor specific lymphocytes with IL-15 and IL-21 for adoptive immunotherapy in melanoma](/preview/png/2116544.png)
Although T Central Memory cells have been described as the most effective T-cell subtype against tumor growth, little is known about the requirements needed for their optimal ex vivo generation. Hence, our goal is to establish a protocol that will lead to consistent ex vivo generation of lymphocytes skewed toward a Central Memory phenotype. Antigen-specific T-cell lines were generated by ex vivo stimulation with Class-I and Class-II melanoma peptide pulsed Dendritic Cells in the presence of either IL-2 or IL-15 plus IL-21. Tumor specific lymphocytes of both Central Memory and effector characteristics were consistently generated from healthy donors and melanoma patients. IL15/IL21 cultures result in a cell population with a lower proportion of CD4+CD25highFoxP3+ regulatory cells and higher number of CD8+ and CD56+ cells, and consequently render a higher yield of cells with a greater cytolytic activity and IFN-γ production against melanoma cell lines.
Journal: Cancer Letters - Volume 285, Issue 1, 18 November 2009, Pages 80–88