کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2120476 1085699 2010 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Apoptosis assays for quantifying the bioactivity of anticancer drug products
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی تحقیقات سرطان
پیش نمایش صفحه اول مقاله
Apoptosis assays for quantifying the bioactivity of anticancer drug products
چکیده انگلیسی

The goal of cancer therapy is to kill cancer cells. Many anticancer drugs are designed to kill cells by inducing apoptosis. However, the potency assays used for measuring the bioactivity of these products are generally cell viability assays which do not distinguish between cell death and growth inhibition. There are a number of commercial assays available to measure apoptosis; however, many of these assays are not appropriate for use in high-throughput screening formats preferred by industry to measure drug activity, also known as potency, due to their inherent low robustness and/or high variability. This review outlines the strengths and weaknesses of current apoptosis assays and highlights new promising assay developments for evaluation of anticancer therapeutics, such as the design of fluorescent and luminescent constructs to be applied as caspase substrates.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Drug Resistance Updates - Volume 13, Issue 6, December 2010, Pages 172–179
نویسندگان
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