کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2145137 1088664 2007 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The alternatively spliced type III connecting segment of fibronectin is a zinc-binding module
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی تحقیقات سرطان
پیش نمایش صفحه اول مقاله
The alternatively spliced type III connecting segment of fibronectin is a zinc-binding module
چکیده انگلیسی

Fibronectin (FN) is a prototypic adhesive glycoprotein that is widely expressed in extracellular matrices and body fluids. The fibronectin molecule is dimeric, and composed of a series of repeating polypeptide modules. A recombinant fragment of FN incorporating type III repeats 12–15, and including the alternatively-spliced type three connecting segment (IIICS), was found to bind Ni2+, Cu2+ and Zn2+ divalent cations, whereas a similar fragment lacking the IIICS did not. Mutation of two pairs of histidine residues in separate spliced regions of the IIICS reduced cation binding to near the level of the variant lacking the IIICS, suggesting a zinc finger-like mode of cation coordination. Analysis of native FNs purified from plasma or amniotic fluid revealed significant levels of zinc associated with those isoforms that contain the complete IIICS. Taken together, these data demonstrate that the IIICS region of FN is a novel zinc-binding module.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Matrix Biology - Volume 26, Issue 6, July 2007, Pages 485–493
نویسندگان
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