کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2145311 | 1088671 | 2008 | 12 صفحه PDF | دانلود رایگان |
The syndecans are a family of cell-surface heparan sulfate proteoglycans consisting of a core protein with covalently attached glycosaminoglycan (GAG) chains. Syndecan-4 expression in skeletal muscle is increased in growth-selected animals during proliferation. Previous studies have suggested that cell-surface heparan sulfate proteoglycans like syndecan-4 are involved in fibroblast growth factor 2 (FGF2) signaling by FGF2 binding to the heparan sulfate chains. Fibroblast growth factor 2 is a potent stimulator of muscle proliferation and an intense inhibitor of differentiation. To investigate the functional contribution of the attached syndecan-4 GAG chains, a turkey syndecan-4 full length cDNA was cloned and mutated at two or all three potential GAG attachment sites at Ser38, Ser65, and Ser67 resulting in all possible one-chain mutants and a no-chain mutant. Turkey satellite cells were transfected with the wild-type syndecan-4, one-chain mutants, no-chain mutant, or the empty vector and assayed for cell proliferation, differentiation, and FGF2 responsiveness. The wild-type syndecan-4, one-chain mutants, and no-chain mutant inhibited cell proliferation and delayed initial differentiation but did not alter FGF2 responsiveness or function through the mitogen-activated protein kinase pathway. There was no difference between the wild-type syndecan-4, one-chain, and no-chain mutants during these stages. These data suggest that syndecan-4 functions in an FGF2-independent manner and the GAG chains attached to the syndecan-4 core protein are not required for syndecan-4 to affect turkey satellite cell proliferation and the initial differentiation.
Journal: Matrix Biology - Volume 27, Issue 7, September 2008, Pages 619–630