کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2146383 1548341 2013 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Induced protein polymorphisms and nutritional quality of gamma irradiation mutants of sorghum
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی تحقیقات سرطان
پیش نمایش صفحه اول مقاله
Induced protein polymorphisms and nutritional quality of gamma irradiation mutants of sorghum
چکیده انگلیسی


• We analyse kafirin protein polymorphisms induced by gamma irradiation in sorghum.
• One mutant with suppressed kafirins in the endosperm accumulated them in the germ.
• Kafirin polymorphisms were associated with high levels of free amino acids.
• Nutritional value of sorghum can be improved significantly by induced mutations.

Physical and biochemical analysis of protein polymorphisms in seed storage proteins of a mutant population of sorghum revealed a mutant with redirected accumulation of kafirin proteins in the germ. The change in storage proteins was accompanied by an unusually high level accumulation of free lysine and other essential amino acids in the endosperm. This mutant further displayed a significant suppression in the synthesis and accumulation of the 27 kDa γ-, 24 kDa α-A1 and the 22 kDa α-A2 kafirins in the endosperm. The suppression of kafirins was counteracted by an upsurge in the synthesis and accumulation of albumins, globulins and other proteins. The data collectively suggest that sorghum has huge genetic potential for nutritional biofortification and that induced mutations can be used as an effective tool in achieving premium nutrition in staple cereals.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis - Volume 749, Issues 1–2, September 2013, Pages 66–72
نویسندگان
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