کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2148148 1548611 2012 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Characterization of synthesized silver nanoparticles and assessment of its genotoxicity potentials using the alkaline comet assay
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی تحقیقات سرطان
پیش نمایش صفحه اول مقاله
Characterization of synthesized silver nanoparticles and assessment of its genotoxicity potentials using the alkaline comet assay
چکیده انگلیسی

Nano-silver (Nano-Ag) particles were synthesized and then characterized using transmission electron microscopy (TEM) and X-ray diffractometry. TEM showed that Nano-Ag were spherical in shape and their size ranged from 40 to 60 nm. X-ray diffractometry indicated that the sample was crystalline and had a face centered cubic structure of pure silver. Genotoxicity of this Nano-Ag was evaluated in human peripheral blood cells using the alkaline comet assay. Results indicated that Nano-Ag (50 and 100 μg/mL) caused DNA damage following a 3 h treatment. Subsequently, a short treatment of 5 min also showed DNA damage. In conclusion, we have shown that the synthesized Nano-Ag induced DNA damage in human peripheral blood cells as detected by the alkaline comet assay. Results further indicated that treatment of cells with Nano-Ag in the presence of hydrogen peroxide did not induce any DNA damage.


► Silver nanoparticles (Nano-Ag; 40–60 nm) synthesized and characterized.
► Nano-Ag produced DNA migration on alkaline comet assay via release of free radicals.
► Nano-Ag decreased the DNA migration induced by H2O2, by neutralizing free radicals.
► Nano-Ag can thus release as well as scavenge free radicals.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Mutation Research/Genetic Toxicology and Environmental Mutagenesis - Volume 742, Issues 1–2, 18 February 2012, Pages 61–65
نویسندگان
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