کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2149214 1089606 2007 13 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
2,4-Dichlorophenoxyacetic acid: Effects on Syrian hamster embryo (SHE) cell transformation, c-Myc expression, DNA damage and apoptosis
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی تحقیقات سرطان
پیش نمایش صفحه اول مقاله
2,4-Dichlorophenoxyacetic acid: Effects on Syrian hamster embryo (SHE) cell transformation, c-Myc expression, DNA damage and apoptosis
چکیده انگلیسی

2,4-Dichlorophenoxyacetic acid (2,4-D) is a selective, systemic auxin-type herbicide extensively used throughout the world. The present research was aimed at studying effects of low and non-cytotoxic concentrations of 2,4-D on SHE cells in relation with carcinogenicity. Effects were studied on Syrian hamster morphological cell transformation, c-Myc expression – both at the gene and protein level – DNA damage and apoptosis.2,4-D significantly induced cell transformation at 11.5 μM and 23 μM (i.e. 2.5μg/mL and 5 μg/mL). An increase in the expression of the transcription factor c-Myc, measured by use of RT-PCR with respect to mRNA level and by Western blotting for protein level was registered at these concentrations, as well as genotoxic effects evaluated with the single-cell gel electrophoresis (Comet) assay. Consequences for apoptosis of 2,4-D treatment were also investigated. The fluorochrome acridine orange was used to study DNA fragmentation as a marker of apoptosis. No effect on apoptosis was found at 2,4-D concentrations that induced cell transformation. This was confirmed by the unchanged expression of Bcl-2 and Bax, two regulator genes of the mitochondrial pathway of apoptosis.Our results demonstrate the transforming and genotoxic effects of low concentrations of 2,4-D in mammalian cells. This information contributes to a better understanding of the mechanism of 2,4-D toxicity in mammalian cells and demonstrates that 2,4-D should be considered as potentially hazardous to humans.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Mutation Research/Genetic Toxicology and Environmental Mutagenesis - Volume 631, Issue 2, 28 July 2007, Pages 124–136
نویسندگان
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